Antigen

Antigen Class

TFs and others

Antigen

CTCF

Cell type

Cell type Class

Blood

Cell type

Monocytes-CD14+

Tissue

monocytes

Lineage

mesoderm

Description

Monocytes-CD14+ are CD14-positive cells from human leukapheresis production, from donor RO 01746 (draw 1 ID is RO 01746, draw 2 ID is RO 01826), newly promoted to tier 2: not in 2011 analysis

Sample

age

30 year

sample type

primary cell

cell type

CD14-positive monocyte

sex

male

dev stage

adult

donor ID

ENCDO544CHQ

lab

Bradley Bernstein, Broad

source

Charles Lee

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

general protocol: https://www.encodeproject.org/documents/be2a0f12-af38-430c-8f2d-57953baab5f5/@@download/attachment/Epigenomics_Alternative_Mag_Bead_ChIP_Protocol_v1.1_exp.pdf

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

38829171

Reads aligned (%)

95.7

Duplicates removed (%)

10.0

Number of peaks

26963 (qval < 1E-05)

hg19

Number of total reads

38829171

Reads aligned (%)

94.8

Duplicates removed (%)

11.2

Number of peaks

26649 (qval < 1E-05)